Expression levels of miR-516a-3p and PTPRD in lung adenocarcinoma. A and B: The expression of miR-516a-3p and PTPRD was assessed in different lung adenocarcinoma cell lines (A549, H1299 and SPC-A1) and human bronchial epithelial cell lines (BEAS-2B and 16HBE). C and D: miR-516a-3p was upregulated and PTPRD was downregulated in lung adenocarcinoma tissues compared with matched adjacent normal tissues. E: Negative correlation between miR-516a-3p and PTPRD expression in lung adenocarcinoma. *P<0.05 vs. BES-2B, 16HBE, the negative control and adjacent normal tissues. miR, microRNA; neg, negative.