Figure 2.

Evaluation of transfection efficiency and miR-516a-3p effect on cell proliferation. A: Observation by fluorescence microscopy following 12 h after transfection. B: The expression of miR-516a-3p in cells transfected with miR-516a-3p mimics, miR-516a-3p inhibitor, or negative control was determined by reverse transcription quantitative polymerase chain reaction assay. C: Knock-down of miR-516a-3p could inhibit cell proliferation of lung adenocarcinoma cells, while cell growth could be promoted by over-expression of miR-516a-3p.