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. 2019 Nov 1;12(11):4222–4231.

Figure 5.

Figure 5

PTPRD may be a target gene of miR-516a-3p. A: The TargetScan alignment of PTPRD’s 3’UTR with hsa-miR-516a-3p indicated the highly conserved regions. B: mRNA expression of PTPRD in lung adenocarcinoma cells (H1299 and SPC-A1) transfected with miR-516a-3p mimics, miR-516a-3p inhibitor or negative control was determined using reverse transcription-quantitative polymerase chain reaction. C: Western blot was performed to measure PTPRD protein expression in H1299 and SPC-A1 cells transfected with miR-516a-3p inhibitor or negative control. D: The luciferase activity of PTPRD 3’UTR-luc-WT was decreased following transfection with miR-516a-3p mimics when compared with the control groups. *P<0.05 vs. Negative control. PTPRD, Protein Tyrosine Phosphatase, Receptor Type D; miR, microRNA; Neg, negative.