Figure 2.

Effect of HMGB1 on receptors expression of RASMCs. A. The primary RASMCs were treated with the indicated concentrations of HMGB1 (100-1000 ng/ml) for 48 h, then these cells were assessed for TLR2, TLR4 and RAGE mRNA expression by RT-PCR. B. The primary RASMCs were treated with HMGB1 for 48 h, then the cell total proteins were extracted, and the TLR2, TLR4, and RAGE protein expression were determined by western blotting. *: P < 0.05 vs. Control cells; #: P < 0.05 vs. the RASMCs treated with HMGB1 100 ng/ml; &: P < 0.05 vs. the RASMCs treated with HMGB1 500 ng/ml. Data were expressed as mean ± SEM from four independent experiments.