Fig. 2.

DNA lysis and clarification.

Panel A shows kinetic of host cell DNA reduction, as measured by qPCR, during cell lysis in the presence of 60 U/mL Benzonase®. Data shown is the mean (points) and range (error bars, if range large enough to render them visible) of duplicate samples. The result for each sample was itself the mean of triplicate assay wells.

Panel B shows comparison of five depth filters (indicated by annotation on the graph) on the basis of the relationship of filtrate turbidity versus cumulative throughput. The same cell lysate, with a pre-filtration turbidity of 70 NTU, was passed through each filter. Initial filtrate measurements obtained with Millistak+® CE50, Clarisolve® CS60HX and Clarisolve® CS20MS filters were clearly inferior to those with Millistak+® HC Pro C0SP and so these were not evaluated beyond the initial filtrate sample collection.