Fig. 4.

Comparison of anion exchange media.

Chromatograms from small-scale anion exchange experiments are shown. In all cases, solid lines indicate A₂₈₀ (left axis) and dashed lines indicate conductivity (right axis). A₂₆₀ data was also collected, with results paralleling the A₂₈₀ data, but is omitted from graphs for clarity.

In panels A-I, each column of graphs represents data from a single type of media, as per column captions. Panels A-F show chromatograms from experiments in which previously purified ChAdOx2 RabG was loaded on Mustang Q Acrodisc, NatriFlo® HD-Q Recon Mini, and CIM-QA 1 mL capsules. In panels A-C. breakthrough was observed and hence dynamic binding capacity was calculated for the Mustang Q and NatriFlo® capsules; breakthrough was not observed after loading 4x10¹³ VP on the (larger) CIM-QA column. Panels D-F show the continuations of the above experiments, in which virus was eluted using a linear gradient of increasing conductivity to allow estimation of elution conditions; the conductivity at the peak of virus elution is shown.

Panels G-I show chromatograms from experiments in which diafiltered lysate containing ChAdOx2 RabG was loaded on Mustang Q Acrodisc, NatriFlo® HD-Q Recon Mini, and CIM-QA 1 mL capsules, followed by step elution.

Panel J presents DBC, virus recovery and HCP reduction data from the experiments shown in Panels A-F. In the case of the CIM-QA column, breakthrough was not reached despite loading 3x10¹³ VP on the 1 mL device.

Panels K-L show chromatograms from experiments in which previously purified ChAd63 ME-TRAP and ChAdOx1 RVF were loaded on Mustang Q Acrodisc capsules and eluted with a linear conductivity gradient, as above.