Table 1.
Product yield and quality.
| Virus | Number of runs and vessel type | Culture volume (litres) | USP yield (VP [by qPCR], per litre of culture) | DSP yield (VP [by spectrophotometry], per litre of culture) | Host-cell protein (ng/mL) | Host-cell DNA (ng/mL) | Residual Benzonase (ng/mL) | A260:A280 ratio | Empty capsid : VP ratio (n = 1 per serotype) | Particle: infectivity ratio |
|---|---|---|---|---|---|---|---|---|---|---|
| ChAdOx2 RabG | 1 (2x Mobius vessels) | 4 | 1.2 × 1014 | 3.60 × 1013 | 5.2 (3.3–8.6) | <0.1 | <0.3 | 1.3 (1.28–1.34) | 0.17 | 103 (85–122) |
| 3 (BioBlu vessels) | 3.2 | 8.3 × 1013 | 4.3 × 1013 | |||||||
| (7.9 × 1013–1.4 × 1014) | (1.7 × 1013–6.4 × 1013) | |||||||||
| ChAd63 ME-TRAP | 2 (Mobius vessels) | 2 | 6.4 × 1013 | 3.1 × 1013 | 3 | <0.1 | <0.3 | 1.39 | <0.1 | 56 (23–88) |
| (3.7 × 1013–9.0 × 1013) | (1.3 × 1013–5 × 1013) | (2.9–3.1) | (1.35–1.42) | |||||||
| ChAdOx1 RVF | 2 (Mobius vessels) | 2 | 5.5 × 1013 | 1.3 × 1013 | 4.9 | <0.1 | <0.3 | 1.38 | <0.1 | 146 (99–192) |
| (3.6 × 1013–7.0 × 1013) | (1.1 × 1013–1.5 × 1013) | (3.1–7.9) | (1.37–1.39) | |||||||
Results of a series of process runs with each virus are presented. Results shown are the median of the indicated number of runs, with the range of results in brackets, with the exception of empty capsid : VP ratio, which was assessed once for each serotype. USP yields were quantified by qPCR at the stage of clarified lysate; for each run, yields values were calculated means of triplicate qPCR reactions. Note that yields shown are per litre of culture (to standardize for the varying volumes of the different bioreactor vessels). Approximate yields per infected cell can be calculated by dividing these values by 109 (based upon cell density of c. 109 cells per litre, after the addition of the feed).