Figure 5.

Activation of the Wnt signaling pathway. A. RT-qPCR was utilized to detect the expression levels of miR-601 and mRNA as they relate to the Wnt signaling pathway. B. Protein expression levels of proteins associated with the Wnt signaling pathway were determined by western blot analysis. C. Quantification of the protein expression data is shown. *P<0.05 cf. the negative control group; #P<0.05 cf. the miR-601 inhibitor + KRT5 group. Data are expressed as the mean ± SD, and data were analyzed using oneway analysis of variance (ANOVA). The experiments were performed in triplicate. KRT5, keratin 5.