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Indirect immunofluorescence test using Alphavirus and Flavivirus specific polyclonal antibodies to identify Chikungunya virus (CHIKV) and Dengue virus (DENV)-2 in C6/36 cell cultures. It is possible to observe a gradual increase in fluorescence (viral load) for both isolates. (A–C) for CHIKV and (D–F) for DENV-2 (in the first, second, and third successive passages, respectively). (G) shows uninfected C6/36 cells used as the negative control. Images at 40X magnification.
