Table 1.

Linear regression statistics.

	Linear Equation a	R2	Deviation from Linearity b	Nicotine (mg/mL) Mean ± SEM			Percent Recovery
				25 Puffs	50 Puffs	75 Puffs
Aerosol (n = 5)	Y = 0.001514X + 0.008830	0.81	NS	0.041 ± 0.006	0.094 ± 0.016	0.118 ± 0.010	8.4%–10.1% c
Smoke (n = 5)	Y = 0.0004714X + 0.009724	0.77	NS	0.022 ± 0.002	0.033 ± 0.003	0.045 ± 0.006	9.8%–14.6% d

^a Slopes for smoke and aerosol are significant (p < 0.0001). ^b As determined by the Runs Test. NS = not significant; SEM = standard error of the means. ^c Percent recoveries based on 233 µL (25 puffs), 465 µL (50 puffs), and 698 µL (75 puffs) of E-liquid containing nicotine (20 mg/mL) added to BHI. For every puff, 9.3 µL of E-Liquid is aerosolized [53]. Final concentrations of nicotine in aerosol are 0.47, 0.93, and 1.40 mg/mL for 25, 50 and 75 puffs, respectively. ^d Percent recoveries based on 1.5 mg (25 puffs), 3.1mg (50 puffs), and 4.6 mg (75 puffs) of nicotine bubbled into 10 mL of BHI. Final concentrations of nicotine in smoke would be 0.15, 0.31, and 0.46 mg/mL for 25, 50, and 75 puffs, respectively. This is assuming that every Marlboro^® cigarette contains 0.92 mg of nicotine [54] and that 15 puffs approximates one cigarette [55].