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. 2019 Dec 4;11(12):2963. doi: 10.3390/nu11122963

Figure 7.

Figure 7

Rd and Rg3 -induced anti-steatotic effect is dependent on mTORC1. (A–C) Primary hepatocytes were incubated with PA (125 μM) and WY14643 (30 μM) for 6 h. The expression of PPARα target genes (A) Acox; (B) Acsl and (C) Hmgcs2 were determined by qRT-PCR. (D) The liver tissue and (E) Hep3b cells were incubated with PA (200 μM) and CRG for 24 h. (F) Primary hepatocytes were incubated with PA (200 μM) and Rapamycin (50 nM) with 2.5 μg/mL Rd or 5 μM Rg3 for 24 h. Lipid accumulation was assessed by BODIPY staining. Original magnification, X 20. * p < 0.05 versus vehicle (Veh), ** p < 0.01 versus Veh, *** p < 0.001 versus Veh, **** p < 0.0001 versus Veh.