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Kinetic analysis of GGT inhibition. EqGGT activity was assayed as reported in Materials and Methods in the presence of 40 mM GlyGly and the indicated concentrations of GpNA (A) or in the presence of 3 mM GpNA and the indicated concentrations of GlyGly (B). Double reciprocal Linweaver–Burk plots were obtained in the absence (closed circle) or in the presence of 5 (closed triangles), 10 (closed squares) or 20 (closed diamonds) µM 5-thio (Panels A, B).
