Table 1.
Profiling of recombinant α-MMC-induced inflammatory responses using inflammatory and autoimmunity PCR Array. THP-1 cells were treated with vehicle control, α-MMC (40 μg/mL) or LPS (1 μg/mL) for 24 h. Results are shown as the fold-change compared to control group. The upregulated genes with fold change >2 are shown. N/A = fold change <2. Results are representative of two independent experiments.
| Symbol | Upregulated Gene Expression | ||
|---|---|---|---|
| Gene Name | Fold Change (>2) | ||
| αMMC | LPS | ||
| BCL6 | B-cell CLL/lymphoma 6 | 4.42 | 8.02 |
| C3 | complement C3 | 13.58 | 15.79 |
| C3AR1 | complement C3a receptor 1 | 6.58 | 14.09 |
| CCL11 | C-C motif chemokine ligand 11 | N/A | 3.91 |
| CCL13 | C-C motif chemokine ligand 13 | 14.79 | 35.68 |
| CCL16 | C-C motif chemokine ligand 16 | N/A | 4.73 |
| CCL19 | C-C motif chemokine ligand 19 | N/A | 8.44 |
| CCL2 | C-C motif chemokine ligand 2 | 79.86 | 435.64 |
| CCL3 | C-C motif chemokine ligand 3 | 36.62 | 188.18 |
| CCL4 | C-C motif chemokine ligand 4 | 112.39 | 806.76 |
| CCL5 | C-C motif chemokine ligand 5 | N/A | 2.78 |
| CCL7 | C-C motif chemokine ligand 7 | 9.93 | 18.16 |
| CCL8 | C-C motif chemokine ligand 8 | 8.58 | 23.23 |
| CCR4 | C-C motif chemokine receptor 4 | N/A | 2.99 |
| CCR7 | C-C motif chemokine receptor 7 | 11.47 | 106.82 |
| CD14 | CD14 molecule | 3.98 | 22.67 |
| CD40 | CD40 molecule | 8.89 | 9.11 |
| CSF1 | colony stimulating factor 1 | N/A | 2.09 |
| CXCL1 | C-X-C motif chemokine ligand 1 | 27.92 | 55.1 |
| CXCL10 | C-X-C motif chemokine ligand 10 | 100.52 | 172.33 |
| CXCL2 | C-X-C motif chemokine ligand 2 | 29.89 | 57.36 |
| CXCL3 | C-X-C motif chemokine ligand 3 | 17.37 | 24.9 |
| CXCL6 | C-X-C motif chemokine ligand 6 | 6.41 | 9.84 |
| CXCL9 | C-X-C motif chemokine ligand 9 | 14.82 | 76.48 |
| FASLG | Fas ligand | 3.32 | N/A |
| IFNG | interferon gamma | 5.77 | 2.45 |
| IL10 | interleukin 10 | N/A | 7.81 |
| IL15 | interleukin 15 | 2.74 | 5.62 |
| IL17A | interleukin 17A | N/A | 2.45 |
| IL1A | interleukin 1 alpha | 4.14 | 5.8 |
| IL1B | interleukin 1 beta | 60.4 | 314.3 |
| IL1R1 | interleukin 1 receptor type 1 | N/A | 2.76 |
| IL1RN | interleukin 1 receptor antagonist | 3.37 | 8.5 |
| IL22 | interleukin 22 | 3.18 | 5.36 |
| IL23A | interleukin 23 subunit alpha | 8.46 | 39.64 |
| IL6 | interleukin 6 | 187.71 | 3140.98 |
| CXCL8 | C-X-C motif chemokine ligand 8 | 135.62 | 186.63 |
| IL9 | interleukin 9 | N/A | 4.19 |
| LTA | lymphotoxin alpha | 7.14 | 3.55 |
| LTB | lymphotoxin beta | 2.57 | 6.95 |
| LY96 | lymphocyte antigen 96 | 5.56 | 13.53 |
| MYD88 | myeloid differentiation primary response 88 | 2.79 | 3.14 |
| NFKB1 | nuclear factor kappa B subunit 1 | 4.65 | 4.14 |
| PTGS2 | prostaglandin-endoperoxide synthase 2 | 3.06 | 5.77 |
| RIPK2 | receptor interacting serine/threonine kinase 2 | N/A | 2.01 |
| SELE | selectin E | N/A | 8.25 |
| TLR1 | toll like receptor 1 | 5.38 | 5.75 |
| TLR3 | toll like receptor 3 | 4.24 | 8.76 |
| TLR6 | toll like receptor 6 | 3.81 | 3.34 |
| TLR7 | toll like receptor 7 | 4.6 | 6.17 |
| TNF | tumor necrosis factor | 3.73 | 12.58 |