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Luminescence response in the PC12 assay for normal cells incubated at 37 °C (A), and cells incubated at 30 °C for 1 h (B) and room temperature (21 °C) for 2 h (C) before detection. Incubating cells at a lower temperature before detection significantly increases response in a temperature-dependent manner but decreases sensitivity to α-CTx MII.
