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PC12 assay screen using (A) 50 µM nAChR agonists (acetylcholine, nicotine, cytisine), and (B) 10 µM nAChR antagonists (α-CTxs MII, ImI, LvIA, PeIA) upon stimulation with ACh, under the optimal growth conditions identified in Figure 3 and Figure 4 (treatment with NGF and nicotine, and incubation at 37 °C). The PC12 assay provided qualitative bioactivity assessment for each agonist and antagonist.
