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. 2019 Nov 26;9(12):289. doi: 10.3390/metabo9120289

Figure 2.

Figure 2

Separation of isomeric compounds using pentafluorophenylpropyl-based UHPLC. Panel (A) XIC (mass tolerance: 1 ppm, ionization: ESI(-)) of 172.09792 m/z in an aminoacylase I deficiency sample showing peaks of N-acetylisoleucine (RT: 4.533 min) and N-acetylleucine (RT: 4.628 min). Panel (B): XIC (mass tolerance: 1 ppm, ionization: ESI(-)) of 172.09792 m/z in a medium chain acyl-CoA dehydrogenase deficiency sample showing peaks of N-acetylleucine (RT: 4.598 min), isohexanoylglycine (RT: 4.686 min) and hexanoylglycine (RT: 4.752 min).