Table 2.
Substrate specificity of the alkaline phosphatase/phosphodiesterase CamPhoD from Cobetia amphilecti KMM 296 *.
| Substrate | Amount of Released Pi, mkM |
|---|---|
| pNPP | 0.52 |
| GTP | 0.475 |
| CMP | 0.465 |
| UMP | 0.37 |
| dCMP | 0.325 |
| AMP | 0.31 |
| TMP | 0.305 |
| CTP | 0.24 |
| GDP | 0.19 |
| GMP | 0.16 |
| UDP | 0.15 |
| CDP | 0.14 |
| dGMP | 0.12 |
| UTP | 0.12 |
| dCTP | 0.09 |
| dAMP | 0.055 |
| TTP | 0.05 |
| dGTP | 0.04 |
| c-di-GMP | 0.005 |
| bis-pNPP | 0.003 |
| 5′-pNP-TMP | 0.002 |
| λ DNA | 0 |
| pUC19 | 0 |
| Oligonucleotides | 0 |
* These results were obtained using a molybdate reagent with ascorbic acid [54]. Various substrates were added to the reaction mixture at 2–15 mM concentrations. For each substrate, a control consisting of a specific substrate and a buffer containing 25 mM Tris-HCl (pH 9.0), 2 mM CoCl2, and 2 mM FeCl3 was used.