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. 2019 Nov 26;11(12):1095. doi: 10.3390/v11121095

Figure 2.

Figure 2

cGAS and STING-mediated sensing of PFV-associated reverse transcription products. PMA-differentiated THP-1 wild type cells and KO variants with deficiencies in components of various innate sensing pathways, as indicated, were incubated with wild type PFV-RCP (MOI 0.2) or pUC19 (mock A) mock supernatants. ISG mRNA levels normalized for RPL13A mRNA levels were determined by qPCR at the indicated time points post exposure. Means ± SEM, plus individual data points, of ISG56 (n = 3) induction normalized to RPL13A relative to mock A treatment are shown. Two-way ANOVA with Tukey’s multiple-comparisons test was used to assess significance. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns: not significant (p ≥ 0.05).