Table A3.
Antibodies used for FACS analysis.
| Antibody Specificity | Company | Clone | Volume per Sample in µL | Fluorophore Attached |
|---|---|---|---|---|
| CD14 | BioLegend | M5E2 | 1 | Pacific Blue |
| CD163 | BD Bioscience | GHI/61 | 2 | PE |
| CD206 | BD Bioscience | 19.2 (RUO) | 2 | APC |
| CD11c | Miltenyi | N418 | 2 | Vio Blue |
| CD1a | BioLegend | SK9 | 2 | PE |
| CD16 | BioLegend | 3G8 | 2 | APC |
| CD86 | BioLegend | IT2.2 | 1.5 | PE |
| IgG2a, κ; isotype control | BioLegend | MOPC-173 | 1 | Pacific Blue |
| IgG1, k Isotype control | Beckman Coulter | Clone GHI/61 (RUO) | 2 | PE |
| IgG1, k Isotype control | BD Bioscience | MOPC-21 | 2 | APC |
| IgG2b, isotype control | Miltenyi | IS6-11E5.11 | 2 | Vio Blue |
| IgG2b, k isotype control | BioLegend | MG2b-57 | 2 or 1.5 | PE |