Skip to main content
. 2019 Nov 22;11(12):1086. doi: 10.3390/v11121086

Figure 5.

Figure 5

The map of NLSs in ORF2. (A) The main motifs of the NLSs in the N-terminal ORF2 (1–38 aa) are bold and the three NLSs are listed. (B) A sketch of the ORF2 indicating putative NLSs motifs by red boxes is shown, and the mutated fragments of the ORF2 are shown. (C) PK-15 cells were transfected with plasmids carrying mutated ORF2 gene (pEGFP-ORF2-∆NLS 2,3, pEGFP-ORF2-∆NLS 1,2, pEGFP-ORF2-∆NLS 1,3, and pEGFP-ORF2-∆NLS 1,2,3) to analyze their subcellular location. Subcellular localization of green fusion proteins was analyzed by confocal microscopy. Scale bars = 20 μm.