Figure 7.

The antiviral activity of IFI16 is dependent on MAVS. (A,B) MARC-145 cells were transfected with siMAVS or siNC for 48 h, the silence efficiency of MAVS was examined by RT-qPCR and western blotting. (C) MARC-145 cells were transfected with siMAVS and IFI16-Myc, and 24 h later, and the cells were infected with PRRSV-2 BJ-4 at a MOI of 0.1 for 24 h. Then, the cells were harvested, and RNA levels of PRRSV were determined by RT-qPCR. (D) The virus titers from cell supernatants were analyzed by TCID₅₀. (E) The expression levels of N protein were detected by western blotting. (F,G) The relative intensity ratios of MAVS and N protein were analyzed using Image J software. All experiments were repeated at least three times with similar results. * p < 0.05, ** p < 0.01.