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Method recovery of pooled urine samples spiked with 0.250 (black bars) or 0.080 ng/mL (gray bars) of MC-RR, MC-LR, or MC-LF prior to IC compared to post-spike controls. % Recovery = concentration of pre-spike sample/concentration of post-spike sample × 100%. Statistical significance of data was determined by one-way ANOVA and Tukey’s multiple comparisons post-test (n = 3).
