Figure 1.

ZR-75-1 cells treated with K092A. (A) Mitochondrial activity measured by using the WST-1 colorimetric assay (OD 450 nm–OD 620 nm) at 6 h, 12 h, 24 h, 48 h, 72 h and 96 hpt under three different conditions of cell culture: culture media (black bars), culture media with 0.01 M ammonium bicarbonate (control, grey bars), culture media with K092A at 1.22 µg/µL (red bars). Data were obtained from three independent experiments (N = 3; n = 6) and statistical analyses were performed using the Mann and Whitney test (p < 0.05 between each a to e statistical groups). (B) Representative light microscopic observations of ZR-75-1 control cells and K092A-treated cells at 72 hpt. Inserts were focused so as to distinguish adherent cells (white arrows) from cells presenting an abnormal morphology (grey arrow) and round suspended cells (black arrow). Both bars represented 40 µm.