Table 1.
Orthogonal design and the responding chitinolytic activity.
| No. | (A) Glucose (g L−1) | (B) Peptone (g L−1) | (C) Chitin (g L−1) | (D) Mg2+ (mM) | Chitinolytic Activity (U L−1) |
|---|---|---|---|---|---|
| 1 | 5 | 1 | 5 | 1 | 52.25 ± 3.73 |
| 2 | 5 | 2 | 10 | 5 | 16.17 ± 2.16 |
| 3 | 5 | 3 | 15 | 10 | 22.64 ± 6.26 |
| 4 | 10 | 1 | 10 | 10 | 18.66 ± 3.73 |
| 5 | 10 | 2 | 15 | 1 | 51.01 ± 2.15 |
| 6 | 10 | 3 | 5 | 5 | 7.46 ± 6.47 |
| 7 | 15 | 1 | 15 | 5 | 72.16 ± 7.77 |
| 8 | 15 | 2 | 5 | 10 | 37.32 ± 3.73 |
| 9 | 15 | 3 | 10 | 1 | 6.22 ± 2.16 |
| K1 | 91.07 | 143.08 | 97.04 | 109.49 | |
| K2 | 77.14 | 104.51 | 41.06 | 95.80 | |
| K3 | 115.71 | 36.33 | 145.81 | 78.63 | |
| k1 | 30.36 | 47.69 | 32.35 | 36.50 | |
| k2 | 25.71 | 34.84 | 13.69 | 31.93 | |
| k3 | 38.57 | 12.11 | 48.60 | 26.21 | |
| Range | 12.86 | 35.58 | 34.91 | 10.29 | |
| Factor order | B > C > A > D | ||||
| Optimization combination | A3 | B1 | C3 | D1 | |