Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 12;17(12):701. doi: 10.3390/md17120701

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Purification of two protease inhibitors, ATPI-I and ATPI-II, from Actinia tenebrosa. (A) SDS-PAGE gel visualization of fractions eluted from gel filtration using Sephadex S-200 resin. Fractions 1–12 were collected according to elution time. Inhibitory fractions are indicated by asterisks. (B) UNOsphere S cation exchange chromatography revealed two distinct inhibitory fractions, labeled “I” and “II”; (C) reverse-phase (RP)-HPLC purification of inhibitor ATPI-I; (D) RP-HPLC purification of inhibitor ATPI-II.