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. 2019 Dec 12;17(12):701. doi: 10.3390/md17120701

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Tissue distribution of ATPI-I in A. tenebrosa. (A) Anatomy of a generalized sea anemone (adapted from Reference [33]), showing the locations of tentacles, acrorhagus, and mesenterial filaments. (B) Tissue distribution of messenger RNA (mRNA) of ATPI-I in A. tenebrosa. ATPI-I mRNA is expressed highly in the mesenteric filaments (green) and also present in acrorhagi (red) and to a lesser extent in tentacles (green). FPKM: fragments per kilobase of transcript per million mapped reads. (C) MALDI-MS imaging color map showing the distribution of ATPI-I across a section of A. tenebrosa, which was (D) subsequently stained with hematoxylin and eosin. Color in (C) corresponds to the signal intensity, and scale bars represent 2 mm.