Figure 5.

ATPI-I shows no loss of inhibitory activity after heat treatment. ATPI-I solution was incubated at 95 °C for 5 min and assayed against 3 nM of trypsin using Ac-YASR-pNA (para-nitroaniline) as substrate (n = 3). Trypsin catalytic rate (ΔOD_{405 nm}) was measured after incubation with four concentrations of untreated and heat-treated inhibitor and is presented as a percentage (%) of the catalytic rate of uninhibited trypsin. ATPI-I showed no loss of inhibitory activity following heat treatment.