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. 2019 Dec 12;17(12):701. doi: 10.3390/md17120701

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Multiple sequence alignment of 14 Kunitz homologs identified in an A. tenebrosa whole-body transcriptome. (A) The sequences of ATPI-I and ATPI-II were aligned with 12 deduced sequences (A03–A14) of Kunitz homologs found in an A. tenebrosa transcriptomic library. Conservation scores were calculated based on physico-chemical properties. The P1 residue is indicated by a red rectangle. (B) The number of positions belonging to each conservation score, which ranges from 0 to 11. (C) Molecular model of ATPI-I is shown in ribbon form and colored by conservation scores from 0 (white) to 11 (blue). The P1 residue Arg16 and six conserved Cys residues are shown in stick mode, with numbering according to their position in ATPI-I.