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. 2019 Dec 12;17(12):701. doi: 10.3390/md17120701

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Predicted model of Kunitz domain from A. tenebrosa and the calculated per-residue contribution to stability (ΔΔG). (A–D) Predicted model of Kunitz domain (shown in ribbon mode) from A. tenebrosa, highlighting residues (shown in stick mode) with conservation level scores of (A) 9–11, (B) 5–8, (C) 2–4, and (D) 0–1. The highlighted residues are colored according to residue properties: non-polar residues are colored white, polar uncharged residues are colored green, basic residues are colored blue, and acidic residues are colored red. (E) The calculated per-residue contribution to stability (ΔΔG) was plotted against degree of conservation for each position.