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. 2019 Nov 27;11(12):1099. doi: 10.3390/v11121099

Figure 5.

Figure 5

BALF1 modulates autophagy through an LIR-like motif. (A) Representative images of GFP-LC3 HeLa cells transfected with EV and plasmids encoding for BALF1-HA or the indicated BALF1 mutants. At 24 h p.t., cells were analyzed by immunofluorescence and observed by confocal microscopy. (B) GFP-LC3 HeLa cells transiently transfected with BALF1-HA. 3D volume rendering (top) and relative IMARIS isosurface 3D rendering (middle) are shown. Scale bar = 5 µm. Magnification of the boxed area is shown in the inset at the bottom (scale bar = 2 µm). (C) Typical LIR sequences were aligned alongside BALF1. Underlined regions indicate highly conserved residues in the putative LIR motif of BALF1. (D) GFP-LC3 puncta were quantified with ImageJ in cells transfected with plasmids encoding for wild-type (wt) or LIR-like mutants of BALF1. Mean ± SEM; n = 50 cells from three independent experiments; ns, not significant; ** P < 0.01.