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. 2019 Dec 1;11(12):2904. doi: 10.3390/nu11122904

Figure 5.

Figure 5

(A) Effect of Calebin A, curcumin, BMS-345541 and specific ASO against NF-κB on TNF-β-induced p65-NF-κB activation in different CRC cell lines. Serum-starved CRC cell lines (HCT116, RKO, SW480) in monolayer cultures were treated as described in Materials and Methods. Immunoblotting of whole cell lysates was performed for anti-phospho-p65. The results are shown from at least three independent experiments and the housekeeping protein β-actin served as an internal loading control. Densitometric evaluation was performed for phospho-p65 for all CRC cell lines. * p < 0.05, ** p < 0.01. (B) Effect of Calebin A on TNF-β-promoted p65-NF-κB activation and NF-κB-regulated gene products involved in proliferation and metastasis in a dose-dependent fashion in different CRC cell lines. Serum-starved CRC cell lines (HCT116, RKO, SW480) in monolayer cultures were treated as described in Materials and Methods. Immunoblotting of whole cell lysates was performed for anti-phospho-p65, anti-integrin-β1, anti-MMP-9, anti-Ki-67, anti-CXCR4 and anti-cleaved-caspase-3. The results are shown from at least three independent experiments and the housekeeping protein β-actin served as an internal loading control. Densitometric evaluation was performed for phospho-p65, β-Integrin, MMP-9, Ki-67, CXCR4 and cleaved-caspase-3 for all CRC cell lines. * p < 0.05, ** p < 0.01.