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. 2019 Nov 28;11(12):1106. doi: 10.3390/v11121106

Figure 1.

Figure 1

Calpain inhibitor prevents the infection by enteroviruses: A549 cells were infected with EV1, CVB3, or CVB1 for 5.5. h p.i. with or without 200 µM calpain inhibitor (ci) or 250 µM elastatinal (ela). (A) Immunofluorescence microscopy was used to evaluate the infection. Viral protein VP1 is in red, and DAPI-stained nuclei are in blue. Scale bar 20 µM. (B) For Western blot analysis, the infected A549 cell lysates were resolved in 4–20% SDS-PAGE gels, blotted, and immunolabeled with antibodies against VP1 and gamma-tubulin as a loading control.