Figure 5.

The alphaherpesvirus envelopment intermediate is non-motile. (a) Naked capsids (red disc) with inner tegument (grey layer) are capable of anterograde traffic along MTs (blue arrow). (b) Following delivery to the envelopment organelle capsids dock and bud into the lumen to assemble infectious enveloped virions. (c) The ESCRT apparatus and the Vps4 ATPase (green cylinder) catalyzes scission at the bud neck to pinch the enveloped virus into the organellar lumen. This envelopment intermediate is non-motile until scission is completed, and can be irreversibly trapped using a dominant negative allele of Vps4 (red text). (d) If scission is successful, the resulting organelle, containing a lumenal enveloped alphaherpesvirus particles, acquires the ability to traffic along MTs.