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. 2019 Dec 10;11(12):1145. doi: 10.3390/v11121145

Figure 4.

Figure 4

Knockdown of AMPK-enhanced PRRSV infection. (A) PK-15CD163 cells were transfected with AMPK-specific siRNAs (siAMPKs) or NC siRNA. At 36 h post transfection, cells were harvested to determine the knockdown efficiency through western blot assay. (B,C) PK-15CD163 were transfected with siAMPKs or NC siRNA. After 24 h, cells were infected with PRRSV (MOI of 0.5) and processed to detect the mRNA levels of the PRRSV NSP9 gene by qRT-PCR assay (B) and virus titers by plaque assay (C) at 24 hpi. The results represent the means and standard deviations from three independent experiments. ***, p < 0.001.