Figure 6.

CP-640186 an ACC1-specific pharmacological inhibitor, inhibited PRRSV infection. (A) PK-15^CD163 cells were incubated with various concentrations of CP-640186 or DMSO as a control for 30 h for MTT assay. (B) PK-15^CD163 cells were pretreated with CP-640186 at various concentrations (0.2, 1, and 5 µM) for 6 h prior to PRRSV infection (MOI = 0.5). The infected cells were cultured in the presence of indicated concentrations of CP-640186 and harvested at 24 hpi for western blot analysis using a specific antibody against PRRSV-N protein, and β-actin was used as a loading control. (C) The distribution of PRRSV-N protein (red) and nuclei (blue) in PRRSV-infected PK-15^CD163 cells was evaluated by confocal microscopy at 24 hpi. (D) Virus titers were detected by TCID₅₀ assay. Data are expressed as means and standard deviations from three independent experiments. *, 0.01 ≤ p < 0.05; ***, p < 0.001.