Table 2.

Polyphasic characterization for aflatoxin production in selected fungi isolates.

SN	ID	Isolates Name	Assession No	aflD (Nor-A)	aflR	aflJ	aflM (ver-1)	Omt-A	YES (NH4 Vapor Test)	(β-CDNRDCA)		TLC	HPLC	Selected Isolates
										Yellow Pigment	UV-Florescence
1	FG1	Aspergillus oryzae	MG647833	POS	NEG	POS	POS	NEG	POS	POS	-	nd	nd
2	FG2	Aspergillus flavus	MG659619	NEG	POS	POS	NEG	POS	POS	POS	-	nd	nd
3	FG3	Aspergillus niger	MG647838	POS	POS	POS	NEG	POS	NEG	NEG	-	nd	nd
4	FG4	Aspergillus flavus	MG659628	POS	POS	POS	POS	POS	POS	POS	±	NEG	POS	**
5	FG5	Aspergillus niger	MG647849	POS	POS	POS	NEG	POS	NEG	NEG	-	nd	nd
6	FG6	Aspergillus terreus	MG647840	NEG	POS	POS	NEG	POS	NEG	NEG	-	nd	nd
7	FG7	Aspergillus flavus	MG659631	POS	NEG	POS	POS	POS	POS	POS	+++	POS	POS	**
8	FG8	Aspergillus tubingensis	MG647844	POS	NEG	POS	POS	NEG	NEG	NEG	-	nd	nd
9	FG9	Aspergillus flavus	MH270531	POS	POS	POS	POS	POS	NEG	NEG	-	NEG	POS	**
10	FG10	Aspergillus flavus	MG647845	POS	POS	POS	POS	POS	POS	POS	+	NEG	POS	**
11	FG11	Aspergillus terreus	MG647846	NEG	POS	POS	NEG	NEG	NEG	POS	++	nd	nd
12	FG12	Aspergillus parasiticus	MG659626	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS	**
13	FG13	Aspergillus amstelodami	MG647851	NEG	POS	POS	NEG	NEG	NEG	NEG	-	nd	nd
14	FG14	Aspergillus flavus	MH270612	POS	POS	POS	POS	POS	POS	POS	++++	POS	POS	**
15	FG15	Aspergillus nomius	MG659621	POS	POS	POS	POS	POS	POS	POS	++++	POS	POS	**
16	FG16	Aspergillus tubingensis	MG647853	NEG	NEG	POS	NEG	POS	NEG	NEG	-	nd	nd
17	FG17	Aspergillus terreus	MG647863	POS	POS	POS	NEG	NEG	NEG	NEG	-	nd	nd
18	FG18	Aspergillus flavus	MG659635	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS	**
19	FG19	Aspergillus flavus	MH270544	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS	**
20	FG20	Aspergillus flavus	MG659673	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS	**
21	FG21	Aspergillus niger	MG647867	POS	POS	POS	NEG	NEG	NEG	POS	++	nd	nd
22	FG22	Aspergillus flavus	MH270559	POS	POS	POS	POS	POS	POS	POS	++	POS	POS	**
23	FG23	Aspergillus flavus	MG659626	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS	**
24	FG24	Aspergillus flavus	MH270559	POS	POS	POS	POS	POS	POS	POS	+/-	POS	POS	**
25	FG25	Aspergillus oryzae	MH270563	POS	POS	POS	POS	POS	POS	POS	+/-	POS	POS	**
26	FG26	Aspergillus flavus	MH270574	NEG	POS	POS	POS	NEG	POS	NEG	-	nd	nd
27	FG27	Aspergillus clavatus	MG647850	POS	POS	POS	NEG	NEG	NEG	POS	-	nd	nd
28	FG28	Aspergillus fumigatus	MG647855	NEG	POS	POS	NEG	POS	NEG	NEG	-	nd	nd
29	FG29	Aspergillus fumigatus	MG647869	POS	POS	POS	NEG	POS	NEG	POS	-	nd	nd
30	FG30	Aspergillus terreus	MG647840	POS	POS	POS	NEG	NEG	NEG	NEG	-	nd	nd
31	FG31	Aspergillus flavus	MH270578	POS	POS	POS	POS	POS	POS	POS	++	POS	POS	**
32	FG32	Aspergillus flavus	MG647868	POS	POS	POS	NEG	NEG	NEG	POS	-	nd	nd
33	FG33	Aspergillus flavus	MH270581	POS	POS	POS	POS	POS	POS	POS	++	POS	POS	**
34	FG34	Aspergillus oryzae	MG659690	POS	POS	POS	POS	POS	POS	POS	+	NEG	POS	**
35	FG35	Aspergillus clavatus	MG647856	POS	POS	POS	NEG	NEG	NEG	POS	-	nd	nd
36	FG36	Aspergillus oryzae	MG659633	POS	POS	POS	POS	POS	NEG	POS	+	NEG	POS	**
37	FG37	Aspergillus terreus	MG647866	POS	POS	POS	NEG	POS	NEG	POS	+	nd	nd
38	FG38	Aspergillus terreus	MG647852	POS	POS	POS	NEG	POS	NEG	POS	-	nd	nd
39	FG39	Aspergillus flavus	MG647857	POS	POS	POS	POS	NEG	NEG	POS	-	nd	nd
40	FG40	Aspergillus flavus	MG659627	POS	NEG	POS	POS	POS	POS	POS	+++	POS	POS	**
41	FG41	Aspergillus parasiticus	MG659687	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS	**
42	FG42	Aspergillus flavus	MG659676	POS	POS	POS	POS	POS	NEG	NEG	-	POS	POS	**
43	FG43	Aspergillus nomius	MH270600	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS	**
44	FG44	Aspergillus ochraceoroseus	MH270530	POS	POS	POS	POS	POS	POS	POS	++	POS	POS	**
45	FG45	Aspergillus flavus	MG647871	POS	POS	POS	POS	NEG	NEG	POS	-	nd	nd
46	Control 1	Aspergillus flavus ATCC	259622TM	POS	POS	POS	POS	POS	POS	POS	+++	POS	POS
47	Control 2	Saccharomyces cereviasiae		NEG	NEG	NEG	NEG	NEG	NEG	NEG	-	nd	nd

Keys: POS = positive amplification/reaction, NEG = negative amplification/reaction, + = fluorescence, - = no fluorescence, +/- = low fluorescence, ++ = mild fluorescence, +++ = high fluorescence, ++++ = very high fluorescence, nd = not detected, ** = selected isolates, TLC = thin-layer chromatography, HPLC = high-performance liquid chromatography, YES = yeast extract sucrose medium, and β-CDNRDCA = β-cyclodextrin neutral red desiccated coconut agar.