Figure 1.

Acidic culture conditions increase migration and invasion of gastric cancer (GC) cells. (A) AGS cells were maintained in normal (pH 7.4) or acidic (pH 6.5) medium, and migration of the cells was assessed by using a wound healing assay at 6 h after scratch. (B) Invasion of cells cultured in normal or acidic medium was assessed by a matrigel-coated transwell assay. After 6 h for AGS and 18 h for SNU601, invaded cells were detected under a microscope (left) and the number of invaded cells was counted (right). * p < 0.05, ** p < 0.01 vs. pH 7.4. Scale bar = 100 μm.