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. 2019 Dec 10;11(12):3023. doi: 10.3390/nu11123023

Figure 4.

Figure 4

Ellagic acid suppresses acidity-mediated expression of snail, twist1, and c-myc. (A–F) AGS (A–C) and SNU601 (D–F) cells cultured in normal or acidic growth medium were further incubated for 24 h without or with 5 μM ellagic acid. Then, mRNA expression of the genes encoding snail (A,D), twist1 (B,E), and c-myc (C,F) was analyzed with real-time PCR. * p < 0.05 vs. no treated pH 7.4; # p < 0.05 vs. no treated pH 6.5. (G,H) AGS (G) and SNU601 (H) cells cultured in normal or acidic growth medium were further incubated for 24 h without or with ellagic acid, then total protein extracts were analyzed by immunoblotting with antibodies against snail, twist1, and c-myc (left). Densitometry calculations for immunoblotting data are shown (right). * p < 0.05 vs. no treated control at pH 7.4; # p < 0.05 vs. no ellagic acid at pH 6.5.