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. 2019 Nov 15;118(1):4–14. doi: 10.1016/j.bpj.2019.10.030

Figure 3.

Figure 3

E. coli growth in the presence of ThT. E. coli growing in MM9 media supplemented with (A) glucose or (B) glycerol at increasing ThT concentration (color map) is shown. ThT concentrations in (A) are 10, 25, 50, 75, 100, 200, and 350 μM and in (B) are 10, 25, 50, 75, 100, 150, 175, and 200 μM. The error bars are standard deviations. (C) Maximum growth rates from (A) and (B) for each ThT concentration are given in red and blue, respectively. Each condition was done at least in triplicate, and error bars are the standard deviation. (D) Iin against time in LB and in (E) MM9 + glucose media is shown. Individual cells are shown in cyan (45 in D and 52 in E from at least nine independent experiments), and the average trace is shown in blue. The imaging conditions and Iex = 10 μM are the same for (E) and (D). The black arrows indicate the intensity peaks. To see this figure in color, go online.