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. 2019 Nov 15;118(1):4–14. doi: 10.1016/j.bpj.2019.10.030

Figure 5.

Figure 5

(A) Average traces of ThT fluorescence (in blue) and motor speed (in black) simultaneously measured in five individual cells (individual cell traces are given in Fig. S6). The shaded areas show the standard deviation, and the motor speed has been normalized to the initial value as described in Materials and Methods. (B) ThT (y axes) and PI (color map) equilibration profile in LB are shown. 25 individual traces are given. (C) Average of ThT (in blue), mKate2 (in red) fluorescence, and cell area (in green) simultaneously measured in 12 individual cells from three independent experiments is shown. The shaded areas show the standard deviation. (D) Equilibration profile of 1 μM ThT in LB is shown. Eight single-cell traces and average trace are given in cyan and blue, respectively. (E) Equilibration profile of 10 μM ThT in LB in the absence (gray shaded area) and presence of epifluorescent illumination (light area) is shown. The dye was flowed in the flow cell for the whole length of the experiment, and imaging conditions in the light area were the same as in Figs. 3 and 5D. 44 cells from eight independent experiments are given. To see this figure in color, go online.