Figure 2.

PirAB toxin-induced accumulation of cellular debris in the digestive tract are of epithelial cells origin. Immunohistochemistry of brine shrimp (A. franciscana) larvae after 12 and 24 h post PirAB^VP toxin challenge. The paraffin sections were treated with anti-cytokeratin polyclonal antibody (Pab), specific to keratin group of fibrous proteins in epithelial cells, then counterstained with haematoxylin. (A): 12 h post- experiment, negative control (no PirAB^VP toxin); (B): 24 h post- experiment, negative control (no PirAB^VP toxin); (C–E): 12 h post-challenge; (F–H): 24 h post-challenge. (C–H): PirAB^VP challenge-induced sloughing that leads to accumulation of cellular debris in the intestinal lumen (arrowhead) is of epithelial cell origin.