Figure 2.

PCV2 Rep rather than PCV1 Rep directly promotes IL-10 production in PAMs. (A) 1 × 10⁶ of PAMs were infected with 100 MOI recombinant adenoviruses rAd-Blank, rAd-Rep1, rAd-Rep2, or Mock infection, and then the expressions of Rep were measured by western blotting. (B,C) IL-10 secretion and mRNA levels were detected by ELISA and Q-PCR, respectively. (D) The activities of il10 promoter were measured in the PAMs co-transfected with the Rep1 protein expression plasmid, Rep2 protein expression plasmid, or blank vector (control) by the dual-luciferase reporter assay system. The data of (A) are representative of three independent experiments. The data of (B–D) are the means ± SEM of three independent experiments. *p < 0.05, **p < 0.01 versus Mock infection at same infection time point (B) or control (D). ^##p < 0.01 versus rAd-Blank-infected cells at the same infection time point (B,C) or Rep1 (D). ^&p < 0.05, ^&&p < 0.01 versus rAd-Rep1-infected cells at the same infection time point.