Skip to main content
. 2019 Dec 10;11(12):1141. doi: 10.3390/v11121141

Figure 3.

Figure 3

PCV2 Rep specifically enhances the activity of the p38-MAPK signaling pathway to promote IL-10 expression in PAMs. (A) PAMs were respectively infected with 100 MOI rAd-Blank (Blank) or rAd-Rep2 (Rep2) for 0 h, 24 h, and 48 h, and the expression and the phosphorylation levels of Akt, ERK, and p38-MAPK were detected by western blotting. (B) PAMs transfected with the specific siRNAs of Akt, ERK, p38-MAPK, or NC were infected with Mock, rAd-Blank, or rAd-Rep2 for 48 h, and the production of IL-10 was measured by the ELISA assay. (C) PAMs were transfected with the same siRNAs as B and then inoculated with 5 MOI PCV2 for 24 h. The production of IL-10 was detected by the ELISA assay. The data of (A) are representative of three independent experiments. The data of (B,C) are the means ± SEM of three independent experiments. **p < 0.01 versus NC siRNA-transfected cells.