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. 2019 Dec 3;11(12):705. doi: 10.3390/toxins11120705

Figure 3.

Figure 3

Effects of curine treatment on macrophage activation 4 h after LPS challenge. Concentrations of IL-1β (A), IL-6 (B) and TNF-α (C) in the supernatants of peritoneal macrophage cultures treated with curine (1 or 10 μM) 4h after the stimulus with LPS (500 ng/mL). Results are expressed as means ± Standard Error of Mean (SEM) of two experiments performed in triplicate. + significant difference (p < 0.05) from the unchallenged cells; * significant difference (p < 0.05) from the untreated LPS-challenged cells. Statistical significance was determined with one-way ANOVA and post hoc Tukey test. Assay range of IL-1β and IL-6: 15.6–1000 pg/mL and TNF-α: 31.3–2000 pg/mL.