Figure 3.

Mapping the differences in local flexibility between RfaH states by HDXMS. (A) Shown is structural mapping of the relative deuterium uptake of different regions of NTD and CTD in the context of the full-length RfaH (αRfaH, PDB: 5OND) or the CTD in isolation (βCTD, PDB: 2LCL). Regions are colored with a gradient from cyan (solvent protected against deuterium exchange) to magenta (solvent accessible). Residues whose deuteron incorporation could not be determined by mass spectrometry are shown in brown. (B) Shown is the relative deuterium uptake for RfaH CTD in its α- and β-states (green and orange, respectively) determined by mass spectrometry. To see this figure in color, go online.