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. 2019 Nov 20;118(1):57–69. doi: 10.1016/j.bpj.2019.11.012

Figure 1.

Figure 1

Membrane potential dependence of the rim intensity due to DiOC6 (3) in single PG/PC (2:8)-GUVs not containing small vesicles. (A) CLSM images of the GUVs that interacted with 2 nM DiOC6 (3) under various membrane potentials are shown. The number above each image shows the membrane potential on the GUV membrane from 0 to −118 mV. The bar represents 20 μm. (B) Dependence of the rim intensity due to DiOC6 (3) on membrane potential is shown. Red ○ 1 nM and blue ▲ 2 nM DiOC6 (3). The mean values of rim intensity and their SEs are shown. (C) Quantitative analysis of the membrane potential dependence of rim intensity. I indicates the rim intensity at φm, I(φm). Red ○ 1 nM, and blue ▲ 2 nM DiOC6 (3). A solid line corresponds to Eq. 5. The mean values of ln (2I/I0−1) and their errors are shown. To see this figure in color, go online.