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. 2019 Nov 20;118(1):57–69. doi: 10.1016/j.bpj.2019.11.012

Figure 2.

Figure 2

Interaction of CF-TP10 with single PG/PC-GUVs containing small GUVs. (A and B) CLSM images of (1) AF647, (2) CF-TP10, and (3) differential interference contrast of a PG/PC (2:8)-GUV interacting with 0.50 μM CF-TP10 under φm = −59 mV (A) and 0 mV (B) are shown. The numbers above each image indicate the interaction time of CF-TP10 with the GUV. The bar represents 30 μm. (C) Change in the FI of the GUV shown in (A) over time is given. Red squares and blue triangles correspond to the FI of the GUV lumen due to AF647 and that of the GUV rim due to CF-TP10, respectively. (D) Effect of membrane potential on Pentry (6 min) is shown. Red ▪, 0.50 μM; blue ▲, 0.40 μM; black ●, 0.30 μM CF-TP10. For each condition, the mean values and SDs of Pentry (6 min) of two independent experiments using 7−13 GUVs each were obtained. To see this figure in color, go online.