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. 2019 Nov 20;118(1):57–69. doi: 10.1016/j.bpj.2019.11.012

Figure 3.

Figure 3

Interaction of CF-TP10 with single PG/PC-GUVs containing LUVs. (A and B) CLSM images of (1) AF647 and (2) CF-TP10 of a PG/PC (2:8)-GUV interacting with 0.50 μM CF-TP10 under φm = −59 mV (A) and 0 mV (B) are shown. The numbers above each image indicate the interaction time of CF-TP10 with the GUV. The bar represents 20 μm. (C) Change in the FI of the GUV shown in (A) over time is given. Red squares correspond to the FI of the GUV lumen due to AF647. Blue triangles and black squares correspond to the FI of the GUV rim and the GUV lumen due to CF-TP10, respectively. (D) Effect of membrane potential on Pentry (6 min) is shown. Blue □, 0.50 μM; blue △, 0.40 μM; blue ○, 0.30 μM CF-TP10. For each condition, the mean values and SDs of Pentry (6 min) of two to three independent experiments using 5−14 GUVs each were obtained. For comparison, the results of the effect of membrane potential on Pentry (6 min), determined using the GUVs containing small GUVs (the same data as Fig. 2D), are indicated with red ▪ 0.50 μM, red ▲ 0.40 μM, and red ● 0.30 μM CF-TP10. (E) Effect of membrane potential on Ilumen (6 min) without pore formation is shown. Red ▪, 0.50 μM; blue ▲, 0.40 μM; black ●, 0.30 μM CF-TP10. For each condition, the mean values and SEs of Ilumen (6 min) of 13−37 GUVs obtained by two to three independent experiments using 5−14 GUVs were obtained. To see this figure in color, go online.