Skip to main content
. 2019 Nov 20;118(1):57–69. doi: 10.1016/j.bpj.2019.11.012

Figure 4.

Figure 4

Dependence of rim intensity due to CF-TP10 in membranes on negative membrane potential. (A and B) CLSM images of (1) AF647 and (2) CF-TP10 of a PG/PC (2:8)-GUV (not containing vesicles) interacting with 0.50 μM CF-TP10 under φm = −59 mV (A) and 0 mV (B) are shown. The numbers above each image indicate the interaction time of CF-TP10 with the GUV. The bar represents 30 μm. (C) Change in rim intensity due to CF-TP10 shown in (A) (red □) and (B) (blue △) over time is given. (D) Dependence of rim intensity due to CF-TP10 in membranes on negative membrane potential is shown. Red ▪, 0.50 μM; green ▲, 0.40 μM; black ●, 0.30 μM CF-TP10. For each condition, the mean values and SEs of rim intensity of 10−20 GUVs obtained by two independent experiments using 5−10 GUVs were obtained. (E) Dependence of normalized rim intensity due to CF-TP10 in membranes on negative membrane potential, which was obtained the data shown in (D), is shown. The symbols denote the same conditions as (D). The error bars indicate the relative errors estimated using the mean values and SEs shown in (D). To see this figure in color, go online.