Fig. 1.

TNFAIP1 is low-expressed in human HCC specimens and cell lines. a. Western blot analysis of TNFAIP1 protein expression in HCC peritumor tissues and tumor tissues, β-actin was used as a loading control. b. The protein expression of TNFAIP1 (normalized according to the expression of β-actin) in peritumor tissues and tumor tissues was quantified (***P<0.001, Student's t-test). c. Representative photograph of TNFAIP1 protein expression in peritumor and HCC tumor tissues. Scale bar, 50 μm. d. Quantitative analysis of the staining score of TNFAIP1 in peritumor tissues and HCC tumor tissues (***P < 0.001, Student's t-test). e. Quantitative analysis of the staining score of TNFAIP1 in different tumor grades of human HCC (**P < 0.01, ***P < 0.001, Student's t-test). f. SigmaPlot software was used to analyze the correlation between the staining score of TNFAIP1 and HCC grade (Pearson's correlation coefficient, −0.6129, ***P<0.0001). g. RT-qPCR was used to analyze the mRNA level of TNFAIP1 in a normal hepatocyte cell line (LO2) and human HCC cell lines (HepG2, Bel7402, Hep3B, SMMC7721 and MHCC97H) (**P < 0.01, one-way ANOVA). h. Western blot analysis of TNFAIP1 protein expression in a normal hepatocyte cell line (LO2) and five human HCC cell lines (HepG2, Bel7402, Hep3B, SMMC7721 and MHCC97H). β-actin was used as a loading control. Data are presented as means ± SEM. P-values were determined by two-tailed Student's t-test or one-way ANOVA (**P < 0.01, ***P < 0.001).